raw sequencing reads mapping Search Results


single-molecule long-read accessible chromatin mapping smac-seq sequencing assay  (SMAC Corp)
90
SMAC Corp single-molecule long-read accessible chromatin mapping smac-seq sequencing assay
SAM-seq probes simultaneously chromatin accessibility and DNA methylation on plant tissues. ( A ) Workflow of the SAM-seq protocol and assessment of m6A-MTase sequence preferences on gDNA (EcoGII-treated gDNA). ( B ) Strand-specific m6A levels of EcoGII-treated genomic DNA before and after normalisation for m6A-MTase preferences over the five A. thaliana chromosomes. Centromeres are depicted as grey boxes. ( C ) UMAP projections of Arabidopsis 12-mer sequences coloured by m6A/A levels of EcoGII-treated gDNA, A content and AT content. ( D ) Density plots and correlation analysis between ONT <t>sequencing</t> of gDNA and SAM-Seq for mCG, mCHG, and mCHH levels. ( E ) Strand-specific SAM-seq m6A levels before and after normalisation for m6A-MTases preferences. ( F ) Metaplot of Arabidopsis genes displaying ATAC-seq accessibility (Lu et al. 2017) (right Y axis) and SAM-seq chromatin accessibility (m6A/A) obtained from independent experiments using distinct m6A-MTases (EcoGII or Hia5) as well as distinct ONT chemistry (R9.4.1 or R10) (left Y axis).
Single Molecule Long Read Accessible Chromatin Mapping Smac Seq Sequencing Assay, supplied by SMAC Corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/raw+sequencing+reads+mapping/pmc11194078-24-38-37?v=SMAC+Corp
Average 90 stars, based on 1 article reviews
single-molecule long-read accessible chromatin mapping smac-seq sequencing assay - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier
sequencing reads mapping, transcriptome annotations and conversion to gene counts  (AltraBio Inc)
90
AltraBio Inc sequencing reads mapping, transcriptome annotations and conversion to gene counts
SAM-seq probes simultaneously chromatin accessibility and DNA methylation on plant tissues. ( A ) Workflow of the SAM-seq protocol and assessment of m6A-MTase sequence preferences on gDNA (EcoGII-treated gDNA). ( B ) Strand-specific m6A levels of EcoGII-treated genomic DNA before and after normalisation for m6A-MTase preferences over the five A. thaliana chromosomes. Centromeres are depicted as grey boxes. ( C ) UMAP projections of Arabidopsis 12-mer sequences coloured by m6A/A levels of EcoGII-treated gDNA, A content and AT content. ( D ) Density plots and correlation analysis between ONT <t>sequencing</t> of gDNA and SAM-Seq for mCG, mCHG, and mCHH levels. ( E ) Strand-specific SAM-seq m6A levels before and after normalisation for m6A-MTases preferences. ( F ) Metaplot of Arabidopsis genes displaying ATAC-seq accessibility (Lu et al. 2017) (right Y axis) and SAM-seq chromatin accessibility (m6A/A) obtained from independent experiments using distinct m6A-MTases (EcoGII or Hia5) as well as distinct ONT chemistry (R9.4.1 or R10) (left Y axis).
Sequencing Reads Mapping, Transcriptome Annotations And Conversion To Gene Counts, supplied by AltraBio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/raw+sequencing+reads+mapping/bio_rxiv__2023__02__15__528594-203-8-13?v=AltraBio+Inc
Average 90 stars, based on 1 article reviews
sequencing reads mapping, transcriptome annotations and conversion to gene counts - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier
library preparation, sequencing, read mapping and counting  (Sequentia Biotech)
90
Sequentia Biotech library preparation, sequencing, read mapping and counting
SAM-seq probes simultaneously chromatin accessibility and DNA methylation on plant tissues. ( A ) Workflow of the SAM-seq protocol and assessment of m6A-MTase sequence preferences on gDNA (EcoGII-treated gDNA). ( B ) Strand-specific m6A levels of EcoGII-treated genomic DNA before and after normalisation for m6A-MTase preferences over the five A. thaliana chromosomes. Centromeres are depicted as grey boxes. ( C ) UMAP projections of Arabidopsis 12-mer sequences coloured by m6A/A levels of EcoGII-treated gDNA, A content and AT content. ( D ) Density plots and correlation analysis between ONT <t>sequencing</t> of gDNA and SAM-Seq for mCG, mCHG, and mCHH levels. ( E ) Strand-specific SAM-seq m6A levels before and after normalisation for m6A-MTases preferences. ( F ) Metaplot of Arabidopsis genes displaying ATAC-seq accessibility (Lu et al. 2017) (right Y axis) and SAM-seq chromatin accessibility (m6A/A) obtained from independent experiments using distinct m6A-MTases (EcoGII or Hia5) as well as distinct ONT chemistry (R9.4.1 or R10) (left Y axis).
Library Preparation, Sequencing, Read Mapping And Counting, supplied by Sequentia Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/raw+sequencing+reads+mapping/pmc08088681-185-2-11?v=Sequentia+Biotech
Average 90 stars, based on 1 article reviews
library preparation, sequencing, read mapping and counting - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier
cage library preparation, sequencing, and read mapping on morexv3 annotation  (DNAFORM Inc)
90
DNAFORM Inc cage library preparation, sequencing, and read mapping on morexv3 annotation
SAM-seq probes simultaneously chromatin accessibility and DNA methylation on plant tissues. ( A ) Workflow of the SAM-seq protocol and assessment of m6A-MTase sequence preferences on gDNA (EcoGII-treated gDNA). ( B ) Strand-specific m6A levels of EcoGII-treated genomic DNA before and after normalisation for m6A-MTase preferences over the five A. thaliana chromosomes. Centromeres are depicted as grey boxes. ( C ) UMAP projections of Arabidopsis 12-mer sequences coloured by m6A/A levels of EcoGII-treated gDNA, A content and AT content. ( D ) Density plots and correlation analysis between ONT <t>sequencing</t> of gDNA and SAM-Seq for mCG, mCHG, and mCHH levels. ( E ) Strand-specific SAM-seq m6A levels before and after normalisation for m6A-MTases preferences. ( F ) Metaplot of Arabidopsis genes displaying ATAC-seq accessibility (Lu et al. 2017) (right Y axis) and SAM-seq chromatin accessibility (m6A/A) obtained from independent experiments using distinct m6A-MTases (EcoGII or Hia5) as well as distinct ONT chemistry (R9.4.1 or R10) (left Y axis).
Cage Library Preparation, Sequencing, And Read Mapping On Morexv3 Annotation, supplied by DNAFORM Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/raw+sequencing+reads+mapping/pmc10762323-62-3-13?v=DNAFORM+Inc
Average 90 stars, based on 1 article reviews
cage library preparation, sequencing, and read mapping on morexv3 annotation - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier

Image Search Results


SAM-seq probes simultaneously chromatin accessibility and DNA methylation on plant tissues. ( A ) Workflow of the SAM-seq protocol and assessment of m6A-MTase sequence preferences on gDNA (EcoGII-treated gDNA). ( B ) Strand-specific m6A levels of EcoGII-treated genomic DNA before and after normalisation for m6A-MTase preferences over the five A. thaliana chromosomes. Centromeres are depicted as grey boxes. ( C ) UMAP projections of Arabidopsis 12-mer sequences coloured by m6A/A levels of EcoGII-treated gDNA, A content and AT content. ( D ) Density plots and correlation analysis between ONT sequencing of gDNA and SAM-Seq for mCG, mCHG, and mCHH levels. ( E ) Strand-specific SAM-seq m6A levels before and after normalisation for m6A-MTases preferences. ( F ) Metaplot of Arabidopsis genes displaying ATAC-seq accessibility (Lu et al. 2017) (right Y axis) and SAM-seq chromatin accessibility (m6A/A) obtained from independent experiments using distinct m6A-MTases (EcoGII or Hia5) as well as distinct ONT chemistry (R9.4.1 or R10) (left Y axis).

Journal: Nucleic Acids Research

Article Title: Simultaneous profiling of chromatin accessibility and DNA methylation in complete plant genomes using long-read sequencing

doi: 10.1093/nar/gkae306

Figure Lengend Snippet: SAM-seq probes simultaneously chromatin accessibility and DNA methylation on plant tissues. ( A ) Workflow of the SAM-seq protocol and assessment of m6A-MTase sequence preferences on gDNA (EcoGII-treated gDNA). ( B ) Strand-specific m6A levels of EcoGII-treated genomic DNA before and after normalisation for m6A-MTase preferences over the five A. thaliana chromosomes. Centromeres are depicted as grey boxes. ( C ) UMAP projections of Arabidopsis 12-mer sequences coloured by m6A/A levels of EcoGII-treated gDNA, A content and AT content. ( D ) Density plots and correlation analysis between ONT sequencing of gDNA and SAM-Seq for mCG, mCHG, and mCHH levels. ( E ) Strand-specific SAM-seq m6A levels before and after normalisation for m6A-MTases preferences. ( F ) Metaplot of Arabidopsis genes displaying ATAC-seq accessibility (Lu et al. 2017) (right Y axis) and SAM-seq chromatin accessibility (m6A/A) obtained from independent experiments using distinct m6A-MTases (EcoGII or Hia5) as well as distinct ONT chemistry (R9.4.1 or R10) (left Y axis).

Article Snippet: Because of the lack of endogenous m6A in most eukaryotes, treatment of chromatin with m6A-MTases followed by ONT sequencing could provide a high-resolution snapshot of chromatin accessibility, as was demonstrated using the single-molecule long-read accessible chromatin mapping (SMAC-seq) sequencing assay in yeast and humans ( ).

Techniques: DNA Methylation Assay, Sequencing